Quantitative resolution of succinate-cytochrome c reductase into succinate-ubiquinone and ubiquinol-cytochrome c reductases.
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چکیده
منابع مشابه
Quantitative resolution of succinate-cytochrome c reductase into succinate-ubiquinone and ubiquinol-cytochrome c reductases.
A purified, active succinate-ubiquinone reductase was prepared from succinate-cytochrome c reductase without damage to ubiquinol-cytochrome c reductase by 1.1% Triton X-100 solubilization at pH 8.0, and calcium phosphate column chromatography in 50 mM Tris-succinate buffer, pH 8.0, containing 30 mM potassium phosphate. Succinate-ubiquinone reductase thus obtained contains ubiquinone and catalyz...
متن کاملCytochrome b 560 ( QPs 1 ) of Mitochondrial Succinate - Ubiquinone Reductase
Mitochondrial succinate-ubiquinone reductase is composed of two parts, a water-soluble succinate dehydrogenase and a two-polypeptide membrane-anchoring protein fraction (QPs). The larger polypeptide of QPs is believed to be associated with cytochrome bas, (QPsl). The structure of QPsl was studied by immunochemistry and molecular cloning and sequencing. Antibodies against QPsl were raised in r...
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The cytochrome b-c1 complex has been “solubilized” and purified to a stage containing 6.5 nmoles of cytochrome b per mg of protein. Seven bands are resolved on a polyacrylamide gel electrophoretic column in sodium dodecyl sulfateP-mercaptoethanol medium. Five of these seven bands have been identified as cytochromes b and cl and a non-heme iron protein. The remaining two bands might be associate...
متن کاملEffect of specific lysine modification on the reduction of cytochrome c by succinate-cytochrome c reductase.
The reduction of cytochrome c by succinate-cytochrome c reductase was studied at very low cytochrome c concentrations where the reaction between cytochrome c1 and cytochrome c was rate limiting. The rate constant for the reaction was found to be independent of ionic strength up to 0.1 M chloride, and to decrease rapidly at higher ionic strength, suggesting that the interaction between cytochrom...
متن کاملInteraction and identification of ubiquinone-binding proteins in ubiquinol-cytochrome c reductase by azido-ubiquinone derivatives.
Various azido-ubiquinone derivatives were synthesized and characterized. 3-Azido-2-methyl-5-methoxy-6-(3,7-dimethyloctyl)-1,4-benzoquinone was found to be suitable for the study of specific interaction between ubiquinone (Q) and protein. It was synthesized with high specific radioactivity and used to identify the Q-binding proteins in purified ubiquinol-cytochrome c reductase. This azido-Q deri...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)68141-x